STAR protocols

Protocol for image-based small-molecule screen to identify neuroprotective compounds for dopaminergic neurons in zebrafish.

Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry.

Protocol to combine brain sections from multiple mice into a single block for spatial transcriptomic analyses.

Efficient bi-allelic tagging in human induced pluripotent stem cells using CRISPR.

A protocol for collection and infusion of cerebrospinal fluid in mice.

A step-by-step protocol for capturing conformational snapshots of ligand gated ion channels by single-particle cryo-EM.

Transcriptional profiling of mouse projection neurons with VECTORseq.

In vivo two-photon microscopy protocol for imaging microglial responses and spine elimination at sites of fibrinogen deposition in mouse brain.